MersV1, Main, Exploration, bibRecord, 003966

Antigenic Heterogeneity of the Hepatitis C Virus NS4 Protein as Modeled with Synthetic Peptides

Identifieur interne : 003966 ( Main/Exploration ); précédent : 003965; suivant : 003967

Antigenic Heterogeneity of the Hepatitis C Virus NS4 Protein as Modeled with Synthetic Peptides

Auteurs : J. C. Chang ; C. Seidel [Allemagne] ; B. Ofenloch [Allemagne] ; D. L. Jue ; H. A. Fields ; Y. E. Khudyakov

Source :

Virology [ 0042-6822 ] ; 1999.

RBID : ISTEX:B1DBFB82C85CD25767D7183DCAB41C99557C629D

English descriptors

Teeft :
- Abbott laboratories, Abbott matrix, Acad, Acid sequences, Amino, Amino acid sequences, Antigenic, Antigenic epitopes, Antigenic reactivity, Antigenic targets, Assay, Bhattacherjee, Blood donors, Central part, Chan, Chang, Choo, Clin, Different genotypes, Different sequences, Disease control, Enzyme immunoassay, Epitope, Genome, Genotype, Genotyped panel, Hepatitis, Heterogeneity, Immunoassay, Immunoreacted, Immunoreactive, Immunoreactivity, Invariant positions, Khudyakov, Major genotypes, Matrix, Mcomish, Natl, Peptide, Present study, Primary structure, Proc, Recombinant, Sequence heterogeneity, Serum samples, Serum specimens, Simmonds, Strong antigenic regions, Synthetic peptides, Viral, Virol, Virus antibodies, Virus genome, Virus genotypes, Virus infection, Zhang.

Abstract

Abstract: The effect of sequence heterogeneity on the immunologic properties of two strong antigenic regions of the hepatitis C virus (HCV) NS4 protein was studied by using a set of 443 overlapping 20-mer synthetic peptides. One antigenic region comprising the cleavage site between NS4a and NS4b (region 5-1-1) was modeled with peptides derived from 73 different known sequences, representing HCV genotypes 1–6. The other antigenic region, designated region 59 and located at the C-terminus of the NS4b protein, was modeled with peptides from 7 known sequences representing genotypes 1–3. All peptides were tested for antigenic reactivity by enzyme immunoassay with a panel of anti-HCV-positive serum specimens representing genotypes 1–5. The data demonstrated that immunoreactive peptides fell into two groups. One group, represented by N-terminal peptides, demonstrated genotype-independent immunoreactivity; the other group, from the central part of region 5-1-1, showed strict genotype specificity. Nineteen peptides from the genotype-independent group strongly immunoreacted with a wide range of serum samples containing antibodies to all 5 HCV genotypes. Twenty-five peptides from the genotype-specific group were found to strongly react with serum containing antibodies only to the genotype from which the peptides were derived. Similar to the N-terminal part of region 5-1-1, peptides derived from region 59 did not show genotype-specific immunoreactivity. Some peptides derived from the central part of region 59 showed very strong and broad antigenic reactivity. Thus, after examining two antigenic regions of the NS4 protein, we identified short sequences that can be used for the efficient detection of either genotype-independent or genotype-specific HCV antibodies.

Url:

https://api.istex.fr/ark:/67375/6H6-609LD7XM-X/fulltext.pdf

DOI: 10.1006/viro.1999.9612

Affiliations:

Allemagne

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The effect of sequence heterogeneity on the immunologic properties of two strong antigenic regions of the hepatitis C virus (HCV) NS4 protein was studied by using a set of 443 overlapping 20-mer synthetic peptides. One antigenic region comprising the cleavage site between NS4a and NS4b (region 5-1-1) was modeled with peptides derived from 73 different known sequences, representing HCV genotypes 1–6. The other antigenic region, designated region 59 and located at the C-terminus of the NS4b protein, was modeled with peptides from 7 known sequences representing genotypes 1–3. All peptides were tested for antigenic reactivity by enzyme immunoassay with a panel of anti-HCV-positive serum specimens representing genotypes 1–5. The data demonstrated that immunoreactive peptides fell into two groups. One group, represented by N-terminal peptides, demonstrated genotype-independent immunoreactivity; the other group, from the central part of region 5-1-1, showed strict genotype specificity. Nineteen peptides from the genotype-independent group strongly immunoreacted with a wide range of serum samples containing antibodies to all 5 HCV genotypes. Twenty-five peptides from the genotype-specific group were found to strongly react with serum containing antibodies only to the genotype from which the peptides were derived. Similar to the N-terminal part of region 5-1-1, peptides derived from region 59 did not show genotype-specific immunoreactivity. Some peptides derived from the central part of region 59 showed very strong and broad antigenic reactivity. Thus, after examining two antigenic regions of the NS4 protein, we identified short sequences that can be used for the efficient detection of either genotype-independent or genotype-specific HCV antibodies.</div>
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Antigenic Heterogeneity of the Hepatitis C Virus NS4 Protein as Modeled with Synthetic Peptides

Antigenic Heterogeneity of the Hepatitis C Virus NS4 Protein as Modeled with Synthetic Peptides

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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